Analytical Biochemistry

A Selection of Molecular Biology Protocols


Hugh Griffin

Institute of Food Research, Norwich Research Park
Colney, Norwish NR4 7UA, United Kingdom

E-mail: Hugh.Griffin@bbsrc.ac.uk


Here are some additional related links

Protocols on the WWW

http://www.almac.net/a/gate/protocols.html

This major Jump Station (directory) of a large number of sites provides on-line protocols and techniques in biochemistry, molecular biology, microbiology, genetics, and cell biology. As well as providing links to the protocol sites, it lists the methods, techniques, and recipes provided by each of the sites. This is a good starting point for anyone looking for a specific protocol. It also includes links to other sites of interest to investigators.
Also on this site:

Mark Strom's Molecular Biology Protocols

http://research.nwfsc.noaa.gov/protocols.html

This excellent collection of molecular biology protocols includes useful Methods Forum where you can submit tips, techniques, questions, and comments; a Bioinformatics section; and a list of on-line journals. The methods are nicely organized into sections, including a section on computer analysis of data. There are numerous methods on DNA purification, transformation, library preparation, sequencing, ogligonucleotides, PCR, and electrophoresis.

Brendan Babb's Collection of Protocols and Recipes

http://www.uct.ac.za/depts/mmi/bbhelp/int.html

This is a comprehensive collection of bacterial, protein, and molecular biology techniques, recipes, and protocols. In addition to the large collection of bacterial methods, DNA and RNA protocols, protein and PCR techniques, this site hosts avery useful set of recipes for general and specific laboratory solutions and buffers. These are arranged in alphabetical order from acrylamide to zinc sulfate.

David Bowtell's Biochemistry, Molecular Biology and Cell Biology Protocols

http://grimwade.biochem.unimelb.edu.au/sigtrans.html

Another great collection of protocols on the web, this contains protocols in biochemistry, molecular biology, and cell biology. It also includes DNA isolation, Southern/Northern blot, in situ hybridization, sequencing, phage purification, PCR, and a collection of yeast protocols.

Jim Brown's Molecular Biology Materials and Methods

gopher://ftp.bio.indiana.edu:70/1m/Molecular-Biology/Materials+Methods

This is the gopher version of Jim Brown's excellent Hypercard stock of common molecular biology laboratory protocols. It contains protocols for sequencing, colony lifts, plasmid isolation, footprinting, end-labeling, hybridization, nick translation, and litigation. Also included are more specific methods on the culture and fermentation of methanogens.

Bruce A. Roe's Molecular Biology Methods

http://dna1.chem.uoknor.edu/protokol_book/protocol_index.html

This is a useful manual of many of the everyday methods used in the average molecular biology laboratory, with emphasis on the techniques for large-scale DNA sequencing protocols and DNA sequencing automation techniques.

Ambros Lab Protocol Collection

http://www.dartmouth.edu/artsci/bio/ambros/protocols/worm_protocols.html

A comprehensive collection of nematode-related protocols and information, this site includes general methods, equipment, microinjection, staining, dauer protocols, mutants, and drugs. This is a valuable resource for anyone working in this area.

University of Maryland Applied Molecular Biology

http://umbc7.umbc.edu/~jwolf/method1.html

General laboratory methods and computer guide. General section includes tips on safety procedures, preparation of buffers and solutions, disposal of chemicals, using a pH meter, working with DNA, and sterile technique. There is a section on keeping laboratory notebooks and a computer user's guide. Although written specifically for the University of Maryland, much of the information provided is generally relevant.

Promega

http://www.promega.com/techdoc.html

As well as details of their products, Promega's web site contains much general data which are of use to scientists. Many vector sequences are available for on-line viewing and as text files which can be downloaded. There are also technical data on subjects such as molecular biology, cell biology, neuroscience, and genetic analysis.

Sigma Chemical Co.

http://www.sigma.sial.com

This site provides information on chemicals, equipment, books, and literature from one of the major laboratory suppliers as well as information on SAF bulk chemicals, Aldrich, Fluka, and Supelco. An additional WWW page at the Sigma site (URL: http://www.sigma.sial.com/sigma/estcst3.htm) lists a wide range of other technical web sites, including virtual library resources, enzymes, proteins, molecular modeling, scientific publishers, and a useful section entitled "Safety and Environment."

Internet for the Molecular Biologist

http://www.ifrn.bbsrc.ac.uk/gm/lab/docs/iftmb.html

This page introduces some of the Internet resources that are of use to researchers working in molecular biology, microbiology, and genetics. Given the breadth of this field, and the rate at which Internet resources grow and change, this cannot be a comprehensive review; the aim is simply to give sufficient information to get new useres familiar with the key resources. The main resources described here offer on-line guides and help documentation that are updated with each improvement to the service offered. Here you will find information on all aspects of the internet (WWW, ftp, gopher, databases, e-mail, newsgroups, etc.) as they relate to molecular biology and other life sciences.

REFERENCE:

Swindell, S.R., Miller, R.R., and Myers, G.S.A. (1996) Internet for the Molecular Biologist, Horizon Scientific Press, Wmondham, UK.

Related Links

If you know of sites you want to have included, please send the URL and a short description to us.

Last modified 9 May 1997

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